Fikosiyanin ma bulu
Kit gimoro
Phycocyanin (C-PC) en gino manyalo nywer e pi, marambulu mayudore ahinya kowuok kuom cyanobacteria kaka Spirulina platensis kod Arthrospira maxima. Otiyo kaka pigment ma ng’iewo ler e fotosintesis to bende onyiso teko mar geng’o gik ma nyalo ketho del, ma geng’o tuoche mag del, kendo ma konyo e geng’o tuoche mag ringruok.
Kido mag kemikol kod gedo:
Kilo mar molekul: ~240 kDa (kido mar auchiel)
Maduong' mar Ywayo: 620 nm (rangi ma rambulu marieny)
Rieny: Owuok e 650 nm (duong' mar kwantum ~0.7)
Kido mag Spektroskopik
| Paramita | Nengo | Ber |
| Koefisien mar rumo (ε620) | 1.54×106 m-1cm-1 | Okang' mar kwan |
| Kinde mar ngima mar ler | ns 2.8 | Kuom motegno mar probe |
| Nyalo mar kwantam | 0.68 ± 0.03 | Tiyo gi pichni |
| Nyalo motegno mar picha | t1⁄2 > seche 8 (ler ma nenore) | Paro mar loso |
Nyalo mar ng’injo:
Pi: Nyalo nyuok ahinya (>50 mg/ml)
Etanol: Nyalo nying' matin (5-10 mg/mL)
Ok nyal ng'injo e gik ma nyalo ng'injo (kaka, klorofom, heksan)
Goyo kod Loso:
10-15% mar pi ma ng’ich: Orito gik ma ngima (duong’: 10-15% kuom gik ma ngima ma otuo).
CO2 mapek ahinya: Omedo ler (>95%) gi 17 MPa, 45°C.
Kromatografi: Oting’o otanda ma omedore (EBA) mar C-PC maler mamalo
Yore mag timo nonro
Lor: Pim mar ler mar UV-Vis
Ng’eyo: PAGE mar pinyno + rangi mar zinc
Gik ma kelo chilo: LC-MS/MS (kwiri mag fiko Tich: Temo mar nyalo mar ywayo radikal mar oksijen
Paramita mag ber
| Rang'iny | Lor (A20/A220) | Aplikes |
| Chiemo | >0.7 | Rangi mapile |
| Gigo mar loso wang' | >2.0 | Gik ma itiyogo e loso gik moko |
| Chiemo mag chiemo | >3.0 | Gik ma medo chiemo |
| Timo nonro | >4.0 | Pim mag kemikol mag ngima |
Ohala
✔ Gima nigi teko mar geng’o tuoche mag del – Odwoko piny radikal ma onge chudo
✔ Gima geng’o ng’ol – Odwoko piny ng’ol ma ok rum
✔ Konyruok mar ringruok – Omedo tije mag limfosit
✔ Rito obwongo – Okonyo ngima mar obwongo
✔ Rangi mar piny – Rangi mar chiemo ma bulu ma FDA oyiego
Aplikes
Gik ma konyo e chiemo: Kapsul, pauda (100-500 milig/odiechieng')
Chiemo ma tiyo: Ais krim, madho (rangi mar bulu)
Gigo mar loso wang': Sirum mag kwedo dongo, krim mag rieny
Nonro mar thieth mar ngima: Probe mag ler, tero yath
Loso mwandu
